Exploration of “Component-Target-Pathway” of Guanxinshutong Caspules in Treatment of Coronary Heart Disease / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the"multi-component, multi-target, multi-pathway" network regulation mechanism of Guanxinshutong caspules (GXST) in treatment of coronary heart disease by network pharmacology method. METHODS: The chemical components of GXST were identified based on liquid chromatography-mass spectrometry (LC-MS), and the oral bioavailability (OB)≥30% and drug likeness (DL)≥0.18 were used as the screening conditions for obtaining active molecular compounds. The targets related to the active compounds were predicted through the Traditional Chinese Medicine Systems Pharmacology database (TCMSP).The relevant targets of coronary heart disease were searched through literature mining and multiple databases and compared with the predicted component targets. Finally, the targets were introduced into the Molecule Annotation System 3.0 (MAS 3.0)to analyze the main biological pathways. RESULTS: Forty-three compounds were identified in GXST based on LC-MS technology and 10 main active compounds were determined through OB and DL screening, such as ellagic acid, cryptotanshinone, and tanshinone. These components affected 26 key targets, such as CLP, LDLR, TNF, et al, and 49 KEGG pathways were involved (P<0.05). CONCLUSION: GXST produces therapeutic effect for coronary heart disease mainly through the TGF-beta, T cell receptors, and MAPK signaling pathway.This study further reveals the characteristics of the multi-component, multi-target and multi-pathway of GXST, and lays a certain foundation for further elucidation of the mechanism of GXST in the treatment of coronary heart disease.