Simultaneous Determination of Multiple Constituents in Panacis Japonici Rhizoma from Different Habitats by UFLC-QTRAP-MS/MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a method for the determination of saponins, amino acids and nucleosides in Panacis Japonici Rhizoma from different habitats by UFLC-QTRAP-MS/MS. METHODS: The chromatographic separation was performed on an XBridge C18 coulumn(4.6 mm×100 mm,3.5 μm) at 30 ℃ with gradient elution of 0.1% formic acid solution-0.1% formic acid acetonitrile, and the flow rate was 0.8 mL•min-1, using multiple-reaction monitoring(MRM) mode. RESULTS: The 33 constituents showed good linearity(r>0.999 0) in the range of the tested concentrations; the precision, repeatability and stability were good; the average recovery rates were between 96.95% and 101.8%, and the relative standard deviations were less than 5%. CONCLUSION: The established method is accurate and reliable, which can be used as a reference for the quality evaluation and control of Panacis Japonici Rhizoma.