Effect of Phospholipid Complex on the Degradation of Geniposide by Rat Intestinal Flora in Vitro / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the effect of geniposide phospholipid complex on the degradation of geniposide by intestinal microflora, which provides the rationale for the design of an appropriate geniposide dosage form.METHODS: The intestinal flora was collected from fresh rat feces. The geniposide and geniposide physical mixtures and their complexes were incubated with the rat intestinal flora in anaerobic conditions, respectively. Samples were taken at 0,2, 4, 6, 8 and 12 h. The content of geniposide was determined by HPLC. The preparative geniposide phospholipid complexes were characterized by DSC and X-ray diffraction.RESULTS: In the physical mixture of geniposide and geniposide phospholipids, geniposide continuously decreased in the first 2 h, while the degradation accelerated at 2 to 4 h and completely degraded at 4 h. In the phospholipid complex group, the content of geniposide was decreased by 67.2% within 6 h, and it was completely degraded at 8 h.CONCLUSION: The geniposide can be completely degraded in the gut microbiota of male SD rats in vitro. After the preparation of the phospholipid complex, the degradation rate of geniposide was slowed down. In the physical mixture group, the presence of phospholipids does not delay the degradation of jasminoidin.