Comparison of the Effects of Different Sterilization Methods on the Contents of Index Components in Baras / 中国药学杂志

ABSTRACT

OBJECTIVE: To compare the contents of the index components in Baras treated by different sterilization methods for choosing the best sterilization method.METHODS: The mixed medicinal powder of Baras were treated by autoclave sterilization, circulating steam sterilization, dry heat sterilization and 60Co radiation sterilization, respectively. The contents of iso-chlorogenic acid A, iso-chlorogenic acid C, quercetin and piperine were determined by HPLC. The influence on the contents of the index components and microbe limitation were analyzed.RESULTS: The total number of bacteria and the counts of mold, yeast count and Escherichia coli in the samples treated by the four sterilization methods were all in compliance with the requirements of Chinese Pharmacopoeia. The total content of four kinds of index components in the samples treated by 60Co radiation sterilization method at dose of 4 kGy was slightly higher than that of others, with a loss rate of 1.21%.CONCLUSION: The 60Co radiation sterilization method is suitable for Baras drying with simple process, short drying time, and the ability to effectively retain all index components.