Microdialysis Recovery of Brimonidine Tartrate in Vitro and in Vivo / 中国药学杂志

ABSTRACT

OBJECTIVE: To evaluate the effects of drug concentration and perfusion rate on the recoveries of self-made linear microdialysis probes for further ocular pharmacokinetic study. METHODS:Brimonidine tartrate was selected as the model drug. The in vitro recovery was determined using positive dialysis and retrodialysis at different perfusion rates and drug concentrations. And the in vivo recovery was determined using retrodialysis method. RESULTS: The microdialysis recoveries of brimonidine tartrate were inversely proportional to perfusion rate,while independent of drug concentration. The positive dialysis and retrodialysis recoveries in vitro were different at 1.0 μL•min-1, but no significant difference at 2.0 and 3.0 μL•min-1. The in vitro recoveries were greater than those in vivo. CONCLUSION: The self-made microdialysis probe has stable recovery and can be used in ocular pharmacokinetic study of brimonidine tartrate.