Establishment of Fluorescence Immunochromatographic Assay for Quantitative Detection of Morphine / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a high sensitivity method for rapid quantitative detection of morphine in the biological samples including serum, saliva and urine. METHODS: With the immunochromatographic lateral flow strip as reaction method, the luminescent lanthanide europium nanoparticles covalently conjugated with morphine monoclonal antibody were adopted as reporters. The morphine antigen and goat anti-mouse antibody were coated at the nitrocellulose membrane separately as the test line and control line. The strip based on competitive inhibition immunoassay principle was detected by the fluorescent reader for rapid quantitative detection of morphine in the biological samples. RESULTS: After experiment optimization, the improved strip could provide the line range 3-3 000 ng·mL-1; precise quality morphine control materials verified CV<10%. There were no cross reactions with amphetamine, methylamphetamine, ketamine and so on; keep the strips at 37 ℃ for 7 d, the performance of the strips did not decline markedly. CONCLUSION: The preliminary established method shows high linearity, precision, specificity and stability. The method could quantitatively detect the morphine in the biological samples in short time. It is supposed to be applied into the grassroots unit.