Chemicalcharacterization of Jinming Tablet by HPLC-DAD/ELSD-ESI-TOF/MS and Study on the Multi-Index Quantitative Fingerprint / 中国药学杂志

ABSTRACT

OBJECTIVE: To analyze and identify the compositions using HPLC-DAD/ELSD-ESI-TOF/MS for establishing the multi index quantatitive HPLC fingerprint of the Jinming Tablet, in order to providing scientific basis for its quality control. METHODS: The Agilent SB C18 column (4.6 mm×250 mm, 5 μm) was used with a mobile phase of acetonitrile-0.2% acetic acid in gradient elution, the flow rate was 0.8 mL•min-1, the sample voume was 30 μL, the column temperature was maintaine at 25 ℃, the detection wavelength was set at 280 nm. ESI-TOF/MS positive and negative ion mode scanning was used to qualitatively analyze the components in methanol water extract from Jinming Tablet. RESULTS: Twenty compounds in Jinming Tablet extract could be primarily identified by HPLC-DAD on-line detection, in which five compounds were determined; based on the analysis of multiple batches of samples, the multi index chromatographic fingerprint was established, meanwhile combined with similarity analysis to achieve the quality evaluation of Jinming Tablet. CONCLUSION: The HPLC-DAD/ELSD fingerprint film analysis method for Jinming Tablet is established, which could provide reference for the quality control of the material basis of other compound.