Highly Efficient Screening Anti-osteoporosis Activity Components of Psoralea corylifolia L. Based on Zebrafish Model Combined with HPLC Capture / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate active constituents of Psoralea corylifolia L. against osteoporosis using chromatography capture combined with zebrafish model. METHODS: High performance liquid chromatography (HPLC) was used for analysis and capture components of Psoralea corylifolia L. including coumarins, flavonoids and bakuchiol, and which anti-osteoporosis activity was evaluated by zebrafish model induced by 25 μmol•L-1 prednisolone, 25 μmol•L-1 prednisolone as model group, and 0.4% DMSO medium as vehicle group. Bone of juvenile zebrafish cultured to 8 dpf (day post fertilization) were stained with alizarin red, and inspected with optical microscope, and bone staining area was quantitative analyzed using image software. RESULTS: The RESULTS showed that coumarins and flavonoids can significantly resist bone loss of zebrafish by prednisolone, while the action of bakuchiol was relative weak with heavy toxicity, high dose of bakuchiol led to zebrafish larval death. CONCLUSION HPLC can system analysis and capture ingredients/components of Psoralea corylifolia L., and zebrafish osteoporosis model can evaluate activity of micro ingredients/components, combination of these two METHODS: can broke through double bottleneck of complex components capture and activity evaluation in vivo. It is expected to realize efficient anti-osteoporosis screening of the whole component of Chinese herbal medicine.