Transfection Efficiency of Chlorinated N-trimethyl Chitosan Nanoparticle on Dendritic Cells / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the effect of chlorinated N-trimethyl chitosan (TMC•Cl) nanoparticle with different modified degrees on the transfection efficiency of mouse marrow-derived dendritic cells (DC) to provide a theoretical basis for the study of DC biological immune vaccine with chitosan (CS) nanoparticle adjuvant. METHODS: TMC•Cl /pEGFP-C1 nanoparticles with different modified degrees were prepared by ion-crosslinking method. Particle size and Zate potential were measured by laser particle size analyzer. Nanoparticle quality was evaluated by enzyme protection experiment. DC were cultured in vitro by a modified Insba method, and TMC•Cl nanoparticles with different degrees of modification were analyzed by in vitro transfection experiments to verify the cytotoxicity and transfection efficiency. RESULTS: The nanoparticle size was (220 ± 15) nm, Zeta potential was (29 ± 5) mV, encapsulation efficiency was 99. 8%, and the transfection efficiency of TMC•Cl nanoparticles on DC in vitro increased by 10% than that of CS nanoparticles. CONCLUSION: TMC•Cl nanoparticles can effectively improve the transfection efficiency of pEGFP-C1 plasmid in DC.