Analysis on Blood and Brain Microdialysis Probes Recovery of N-acetyl-L-glutamine, Glutamic Acid and γ- Aminobutyric Acid in Vitro Using LC-MS /MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To determine the recovery rates of blood and brain microdialysis probes of acetylglutamine(NAG) and its decomposition products glutamic acid (Glu) and γ-aminobutyric acid (GABA) in vitro, and lay the foundation for the pharmacokinetic study of NAG in blood and brain. METHODS: The concentrations of NAG, Glu and GABA in blood and brain microdialysates were determined by LC-MS /MS and the probe recoveries were calculated. The in vitro recoveries of NAG, Glu and GABA were studied using positive dialysis and retrodialysis methods at different flow rates and concentrations. RESULTS: The in vitro recoveries of blood and brain probes of NAG, Glu and GABA were inversely proportional to the perfusate flow rates while independent of drug concentrations. The recovery rates obtained by positive dialysis and retrodialysis methods in vitro were approximately equal under the same condition, and were stable for at least 6 h. CONCLUSION: Retrodialysis method can be used to study the probe recovery of NAG, and microdialysis can be used for blood and brain pharmacokinetic study of NAG.