Quantitative Analysis of Multi-Components by Single Marker for Determination of Three Atractylenolides in Atractylodis Macrocephalae Rhizoma / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a quantitative analysis of multi-components by single marker(QAMS) for the determination of three atractylenolides in Atractylodes macrocephala Koidz. METHODS: UPLC method was applied to determine atractylenolide Ⅰ, Ⅱ and Ⅲ by external standard method (ESM) first. With this established UPLC method, atractylenolide Ⅲ was used as the internal standard (IS) to determine the correction factors (RCFs) of the two other atractylenolides, and their contents in all the samples were calculated by their RCFs at the same time. By comparing the contents determined by the ESM and QAMS methods, the feasibility and accuracy of QAMS method were verified. RESULTS: Within a certain range(atractylenolideⅠ 3.7 - 59.3 μg•mL-1, atractylenolide Ⅱ 3.9 - 63.5 μg•mL-1, atractylenolide Ⅲ 7.3 - 116.1 μg•mL-1), the RCFs of atractylenolide Ⅰ, Ⅱ to atractylenolide Ⅲ were 0.633 and 1.895, respectively, which had good repeatability in different experimental conditions. There was no significant difference between the QAMS method and ESM method(P > 0.05). CONCLUSION: The QAMS method is feasible and accurate for the determination of the three atractylenolides and is beneficial to the quality control of Atractylodes macrocephala Koidz..