Preparation and Anti-Leukemia Efficacy of Daunorubicin-Loaded Microparticles / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 526-530, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-858379
ABSTRACT
OBJECTIVE:
To construct a novel daunorubicin-loaded microparticles (DNR-MPs) drug delivery system, and study its proliferation inhibiton effect on leukemia cells.METHODS:
Both DNR-MPs-IDL(DNR microparticles with intracellular drug loading method) and DNR-MPs-EDL (DNR microparticles with extracellular drug loading method) were prepared from HL-60 cells, and the average particle size, Zeta potential and drug loading efficiency of DNR-MPs were measured. The uptake of DNR-MPs by HL-60 cells was analyzed by confocal laser scanning microscopy (CLSM) and flow cytometry (FCM). The inhibition effect of DNR-MPs on the proliferation of HL-60 cells was evaluated by MTT assay.RESULTS:
There was no significant difference in the average particle size and Zeta potential of the two DNR-MPs. The drug loading efficiency of DNR-MPs-EDL was higher compared with DNR-MPs-IDL. The uptake results showed that the two DNR-MPs significantly increased the drug uptake compared with free DNR (P < 0.05). DNR-MPs- IDL showed a higher drug uptake by the cells than DNR-MPs-EDL did (P < 0.05). They also exhibited an enhanced inhibition effect on the proliferation of HL-60 cells compared with DNR (P < 0.05).CONCLUSION:
The different ways of preparation of drug-loaded microparticles can cause varying anti-tumor effect. Microparticles can significantly augment the anti-leukemia efficacy of DNR, indicating a promising drug carrier for the therapy of leukemia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2018
Type:
Article
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