Regulation Effect of Glycyrrhetinic Acid on the Expression of BLM and Proliferation Apoptosis and Invasion Migration of PC3 Cells / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the effects of glycyrrhetinic acid on the proliferation, invasion, migration and apoptosis of PC3 cells and the expression of BLM and Caspase-3. METHODS: The cell proliferation, invasion, migration and apoptosis were monitored by MTT assay, Transwell, scratch and Hoechst/PI double staining method. The transcription levels of BLM and Caspase-3 gene were tested by Real-time quantitative PCR, as well as their expression levels of protein were detected by Western blot. RESULTS: MTT results revealed that the effect on cell proliferation was not significant when the concentration of glycyrrhetic acid under 0.12 mol•L-1. The proliferation of the cell was gradually decreased when the concentration of glycyrrhetic acid is greater than 0.12 mol•L-1. The results of invasion and migration of the cells showed that it is decreased obviously when the concentration of glycyrrhetinic acid was above 0.16 mol•L-1. Hoechst/PI double staining showed that the cell apoptosis was obvious when the concentration of glycyrrhetinic acid was above 0.12 mol•L-1. Fluorescence quantitative PCR results showed that the expression of the BLM gene decreased significantly and the expression of the Caspase-3 gene increased significantly in PC3 cells compared with the control group when the concentration of glycyrrhetinic acid was above 0.12 mol•L-1. Western-blot results showed that BLM protein expression was significantly reduced while the expression of the Caspase-3 protein was significantly increased when the concentration of glycyrrhetinic acid in 0.12 mol•L-1. CONCLUSION: Glycyrrhetinic acid inhibited the proliferation, invasion and migration of the PC3 cells is related to the low expression of the BLM, whereas promoted apoptosis of the PC3 cells and is related to the high expression of Caspase-3.