Effect of 12-Deoxyphorbol-13-Palmitate on Proliferation Inhibition and Apoptosis Induction of HL60 Cells / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 30-34, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-858464
ABSTRACT
OBJECTIVE:
To investigate the effects of 12-deoxyphorbol-13-palmitate (DP) on the proliferative inhibition and induction apoptosis of human acute myelocytic leukemia cell line HL60.METHODS:
HL60 cells were treated with different concentrations of DP. The morphological changes were observed with optical microscope. The effect of DP in cell proliferation was tested using cell counting kit-8(CCK-8) assay, to analyze the cell cloning capacity by methyl cellulose colone-forming experiment. DNA agarose electrophoresis method was used to detect apoptosis. The expression of pro-and anti-apoptotic genes Bcl-2 and Bax were examined by reverse transcription-polymerase chain reaction (RT-PCR).RESULTS:
CCK-8 METHODS and methyl cellulose colone-forming experiment indicated DP had an certainly inhibitory effect on HL60 cells(P<0.05, P<0.01). The characteristics of apoptosis was presented under optical microscope. DNA agarose electrophoresis showed the obvious DNA 1adder. In accordance with RT-PCR experiments, compared with the control group(0 mg·L-1), DP could down-regulate the gene expression of Bcl-2 and up-regulate the gene expression of Bax in HL60 cells(P<0.01).CONCLUSION:
DP can inhibit the proliferation and induce the apoptosis of cultured HL60 cells in a dose-effect and time-effect relationship in vitro, and its mechanism may be related to down-regulating gene expression of Bcl-2, up-regulating gene expression of Bax, rising Bax/Bcl-2 value.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2018
Type:
Article
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