Determination of Free Amino Acids in Hericium erinaceus Mycelium by HPLC with Precolumn Derivatization / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a quantitative method of determining the contents of 15 kinds of free amino acids in the Hericium erinaceus mycelium. METHODS: The HPLC analysis was performed after derivatization by using phenyl isothiocyanate (PITC) as a derivative reagent. The chromatographic column was Ultimate Amino Acid(4.6 mm×250 mm, 5 μm). The mobile phase A was acetonitrile-water (80∶20), and mobile phase B was sodium acetate buffer (pH 6.5). Gradient elution was performed at the flow rate of 1.0 mLmin-1. The detection wavelength was set at 254 nm, and the column temperature was maintained at 40℃. RESULTS: The correlation coefficients of 15 kinds of free amino acids ranged from 0.999 7 to 0.999 9. The average recovery rate (n=6) was between 96.83%-98.60%, and the RSD was between 0.67%-2.67%. CONCLUSION: This method is simple, accurate and can be used to determine the contents of 15 kinds of free amino acids in the Hericium erinaceus mycelium.