Correlation between Expression of LEA2 Gene from Gastrodia elata and Cold Resistance / 中国药学杂志

ABSTRACT

OBJECTIVE: To find out the correlation between LEA2 gene from Gastrodia elata and cold resistance. METHODS: Two LEA2 cDNA sequences were obtained by screening the transcriptome of seeds from Gastrodia elata. Recombinant plasmid was obtained and imported into Escherichia coli. Then the transformed Escherichia coli(pET28a-LEA2) received low temperature treatment. RESULTS: GeLEA2-1 had a complete 477 bp ORF encoding 158 amino acids and its molecular weight was 17.3×103. In addition, GeLEA2-2 had a complete 942 bp ORF encoding 313 amino acids and its molecular weight was 34.85×103. Phylogenetic analysis showed the two GeLEA2 were highly homologous with LEA2 from Arabidopsis thaliana and Oryza sativa. The growth curves of E. coli BL21(DE3) showed that the strain expressing GeLEA2 tolerated low temperature more poorly than the control. CONCLUSION: Our work reveals that GeLEA2 could response to low temperature stress by negative regulation.