Study on Alkine Hydrolysis Kinetics of Capilliposide B and C / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the hydrolysis kinetics of capilliposide B and capilliposide C under different temperatures and pH values, and identify the hydrolysis products. METHODS: The LC-B and LC-C were quantified by HPLC, then the hydrolysis process was deduced. Samples were taken to investigate the stability of LC-C and LC-B at different time points under the conditions of 30, 50, 70℃, and pH 8.0, 9.0, 10.0, 11.0, respectively. The hydrolysis products were identified by mass spectrometry and nuclear magnetic resonance spectroscopy. RESULTS: The hydrolysis reaction rates of LC-B and LC-C obeyed first-order kinetics under different pH values and temperatures. And they were related to hydrolysis temperature and pH at the same temperature, the higher the pH, the faster the hydrolysis rate; at the same pH, the higher the temperature, the faster the hydrolysis rate. The hydrolysis product was capilliposide A. CONCLUSION: The study provided an effective method for the preparation of LC-A.