Intestinal Absorption Characters of Mosapride Citrate Using Caco-2 Cells and Single-Pass Intestinal Perfusion Methods / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the intestinal absorption characters of mosapride citrate(MC) and its tablets. METHODS: The Caco-2 cell monolayer was cultured and the in situ single-pass intestinal perfusion(SPIP) in rat model was created for studying the drug absorption properties, using phenol red method to revise the perfusate volume, a HPLC method was developed to simultaneously detect the phenol red and MC, the absorptive coefficient of Papp and Peff was calculated. RESULTS: MC was absorbed by the whole intestine segments in rats, mainly at upper small intestine. The tablets showed high permeability and good intestinal absorption in Caco-2 cells and SPIP in rats. CONCLUSION: In the Caco-2 cells and rats SPIP models, the tablets are prepared showed good consistency with the branded drug.