Analysis of Antioxidant Activity and Active Components of Phellinus robustus / 中国药学杂志

ABSTRACT

OBJECTIVE: To determine the antioxidant activities of different extracts from Phellinus robustus (PR) and analyze the active components of the extract with the highest antioxidant activity. METHODS: PR was first extracted by ethanol and then the ethanol extract was sequentially partitioned into four fractions. To determine the antioxidant abilities of different extracts, the contents of total phenols and total flavonoids, scavenging activity on DPPH and ABTS radicals, reducing power and inhibition of lipid peroxidation were measured. RESULTS: The ethyl acetate fraction (EAF) showed the highest contents of total phenols and flavonoids and the largest antioxidant capacity, meanwhile, its DPPH radical scavenging activity was comparable to that of the positive control, ascorbic acid (P>0.05). TPC and TFC showed strong positive correlations with FRAP (r2=0.984, P<0.01). Three phenolic acids (protocatechuic acid, vanillic acid and caffeic acid) had been isolated and identified from EAF by HPLC-ECD, and anilic acid and caffeic acid were first isolated from PR. CONCLUSION: EAF from PR has strong potential to be a source of phenolic compounds with antioxidant proprieties and a promising source of food and drugs.