The mRNA Expression Change of BKCa Channel in Renal Artery in T2DM Nephropathy Rats / 中国药学杂志

ABSTRACT

OBJECTIVE: To reveal the mRNA expression change of BKCa channel in renal artery in different course in T2DM nephropathy rats. METHODS: T2DM nephropathy models were induced by one side nephrotomy, high-fat diet in rats and small doses of streptozotocin through intraperitoneal injection; Real-time PCR were performed to verify mRNA expression. Then body weight, 24 h urinary albumin, blood urea nitrogen(BUN), serum creatinine(Scr), kidney weight are measured. Renal tissue was observed under optical and electron microscope. RESULTS: Blood glucose, blood insulin, serum creatinine, urea nitrogen, 24 h urinary albumin, kidney hypertrophy index of T2DM nephropathy rats in 8 weeks and 12 weeks, were significantly higher than that of control group (P<0.05, P<0.01);the rat kidney pathological morphological changes were obvious. Compared with the control group, ɑ subunit expression of BKCa in the model group at 8 weeks and 12 weeks were obviously unchanged;β1 subunit expression of BKCa were reduced(P<0.05, P<0.01). CONCLUSION: The mRNA expression of BKCa are reduced in renal artery in different course, and this chang is consistent with the pathological changes degree of kidney in T2DM nephropathy rats.