Effects of Lappaconitine Sulfate on Proliferation, Cycle and Apoptosis of Human Cerical Neoplasm Hela Cells / 中国药学杂志

ABSTRACT

OBJECTIVE: To observe the effects of lappaconitine sulfate on proliferation, cycle and apoptosis of human cerical neoplasm Hela cells and its possible mechanism. METHODS: Cell Counting Kit-8 (CCK-8) was adopted to observe the inhibitory effects of lappaconitine sulfate on the proliferation of Hela cells and calculated its half maximal inhibitory concentration (IC50). The technology of flow cytometry, propidium iodide (PI) single stained method and Annexin-V-FITC/PI double stained method were employed to measure the cell cycle and apoptosis of Hela cells after lappaconitine sulfate treatment. RESULTS: Lappaconitine sulfate could inhibit the proliferation of human cerical neoplasm Hela cells in vitro, and the inhibition presented obvious dose-effect relationship (r=0.990 5, P<0.01). The IC50 of lappaconitine sulfate on Hela cells was 0.421 mg·mL-1. After dealt with lappaconitine sulfate, Hela cells indicated remarkable cell cycle arrest at G0/G1 phase, and the apoptotic ratio was gradually increased with the concentration of lappaconitine sulfate. CONCLUSION: Lappaconitine sulfate could inhibit the growth of human cerical neoplasm Hela cells, change the distribution of cell cycles and induce their apoptosis.