Simultaneous Determination of Contents of Seven Tannins Active Ingredients in Chebulae Fructus Retz from Different Areas by HPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop an HPLC method for simultaneous determination of seven tannins in Chebulae Fructus, including gallic acid, chebulic acid, corilagin, ethyl gallate, ellagic acid, chebulagic acid and 1, 2, 3, 4, 6-O-pentagalloylglucose and determine the contents of the seven tannins in Chebulae Fructus Retz from different areas. METHODS: The HPLC analysis was carried out on an Hypersil ODS2 C18 (4.6 mm×250 mm, 5 μm) column with acetonitrile (A) and 0.05% formic acid solution in water (B) as mobile phase in a linear gradient elution mode. The UV detection wavelength was set at 290 nm and the flow rate was 1.0 mL·min-1. RESULTS: The calibration curves of the seven tannins all showed good linearity (r>0.999 8). The recovery rates were in the range of 95.2% to 98.4%. All the seven tannins could be detected in the two kinds of Chebulae Fructus Retz from eight regions, but the amounts of these tannins varied significantly. The contents of the seven tannins active ingredients in Chebulae Fructus of Terminalia chebula Retz from Hainan, Guangxi, Guangdong and Xinjiang were much higher than those from other areas, while those in Chebulae Fructus of Terminalia chebula Retz. var. tomentella Kurt were higher in Guangdong and Guangxi than other areas. CONCLUSION: The method is proved to be accurate and valid, and can be used for the quality control of Terminalia chebula Retz.