Purification and Monosaccharide Composition Analysis of Polysaccharides from Pteridium revolutum and Effects on Macrophage RAW264.7 / 中国药学杂志

ABSTRACT

OBJECTIVE: To purify polysaccharides from Pteridium revolutum (Blume.) Nakai and determine their relative molecular weights, monosaccharide compositions and immunomodulating activities. METHODS: Crude polysaccharide was extracted from P. revolutum with hot water and then purified by DEAE Sepharose Fast Flow and Sephadex G-200 chromatography column after removing proteins by Seveg method. The immunomodulating activities of the polysaccharides were investigated on macrophage cells RAW264.7. RESULTS: Three homogenous polysaccharides, PRP0, PRP1 and PRP2, were obtained from the crude polysaccharide. Their molecular weights were 1.04×106, 8.39×105 and 7.37×105, respectively. Monosaccharide composition analysis by gas chromatography revealed that all three homogenous polysaccharides were composed of arabinose, fucose, galactose, mannose, glucose, rhamnose, and xylose in different ratios. The crude polysaccharide and three homogenous polysaccharides could promote the proliferation of RAW264.7 cells at the concentration of 12.5-100 μg·mL-1 and significantly induce the production of NO from RAW264.7 cells in a dose-dependent manner. CONCLUSION: P. revolutum polysaccharides can improve immunity activity.