Identification of Ophiopogon japonicus from Sichuan by Polymerase Chain Reaction / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 936-940, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-858690
ABSTRACT
OBJECTIVE:
To design specific PCR primers and establish the PCR identification method of Ophiopogon japonicas from Sichuan.METHODS:
The gene footprint of Ophiopogon japonicas from Sichuan named CM503 was screened from random amplified polymorphic DNAC(RAPD) amplification. Reclaimed CM503 gene was inserted into T-vector to be cloned and sequenced. One pair of specific primers CM1/CM2 were designed according to the CM503 sequence and applied in specific PCR using the genomic DNA of Ophiopogon japonicas from Sichuan as template.RESULTS:
A specific band around 297 bp was detected in Ophiopogon japonicus from Sichuan at 68℃, while nothing appeared for the other varieties.CONCLUSION:
The method is convenient, reproducible, and precise, with broad application prospects.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2017
Type:
Article
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