Inhibition of the Inflammatory Response and Its Mechanism by Hydrochloric Acid Chlorobenzene Sulperzon (HACS) in Astrocytes / 中国药学杂志

ABSTRACT

OBJECTIVE: To evaluate the inhibitory effects and mechanism of Hydrochloric acid chlorobenzene sulperzon (HACS) on neuronal inflammation were studied in order to evaluate possible application of it in AD therapy. METHODS: The release of NO (nitric oxide) by astrocyte was detected by Griess methods and the chemotaxis of mouse macrophage was detected by Boyden chemotaxis chamber. The cell viability was detected by MTT assay. The content of IL-6 and RANTES (T cell expressed and presumably secreted)was determined by ELISA. The expression of mRNA of IL6 and RANTES was detected by RT-PCR. The intracelluar Ca2+ was detected by confocal microscope. RESULTS: HACS efficiently decreased the release of NO from astrocyte stimulated by LPS (1 μg·mL-1), chemotaxis of mouse macrophage stimulated by PAF (5×10-8 mol·L-1), expression of IL-6 and regulated upon activation of RANTES in U251 cells induced by IL-1β (50 ng·mL-1). In addition, HACS significantly inhibited the increase of intracellular Ca2+ in U251 cells induced by Aβ1-42(50 μg·mL-1)/sodium glutamate(100 μmol·L-1)or IL-1β(50 ng·mL-1). CONCLUSION: HACS efficiently inhibites the activation of astrocyte by regulation of intracelluar Ca2+inhibition of chemotaxis and decrease of inflammatory cytokines. Especially, the inhibition of RANTES and intracelluar Ca2+ induced by inflammatory mediators by HACS is firstly reported in this study.