Effect of Andrographolide Derivative ADA on Apoptosis and Its Mechanism of Breast Cancer MCF-7 Cells / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the anti-proliferation effects of andrographolide Derivative(ADA) and its potential anti-tumor mechanisms in MCF-7 cells. METHODS The anti-proliferation effects of ADA were assessed by MTT assay in MCF-7 cells. Apoptotic morphological changes of MCF-7 cells after ADA treatment were observed by high content screening (HCS) after AO/EB double staining and DAPI, Apoptotic number was investigated by using flow cytometry. The expression of Bcl-2, Bax, caspase 3, NF-κB p65, p-IκBɑ and p-IKKβ were measured using Western blot. RESULTS: MTT assay demonstrated that ADA exerted potent anti-tumor effect in a significant concentration-dependent manner;ADA could promote the apoptosis of MCF-7 cells. Western blot results showed that Bcl-2 families and NF-κB p65 and caspase 3 involved in the process of apoptosis, Bcl-2 expression lowered(P<0.05), p-IκBɑ and p-IKKβ expression decreased(P<0.01), Bax, NF-κB p65 and caspase 3 expression increased(P<0.01). CONCLUSION: ADA could inhibit the proliferation of MCF-7 cells and mechanism may be that it might induce apoptosis by regulated the expression of Bax, Bcl-2 and caspase 3 protein and NF-κB passway.