Determination of Six Active Components in Dengyinnaotong Capsules by HPLC-MS/MS / 中国药学杂志

ABSTRACT

OBJECITVE To establish an HPLC-MS/MS method for simultaneous determination of six active components in Dengyinnaotong capsules, ie, scutellarin, bilobalide, ginkgolide A, ginkgolide B, ginsenoside Rg1 and notoginsenoside R1. METHODS: The chromatographic separation was carried out at 30℃ on a Phenomenex Luna C18 (4.6 mm×150 mm, 5 μm) column eluted by gradient program. The flow rate was 0.3 mL·min-1. The six compounds were separated within 10.0 min. RESULTS: The regression curves for the six compounds showed good linearity in wide ranges. The recoveries were around from 94.8% to 108.5%. CONCLUSION: The established method is accurate, reliable, specific and reproducible, which can be used for the quality control of Dengyinnaotong Capsules.