Quantification of the major bile acids in bear bile powder by HPLC-ELSD / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an HPLC-ELSD method for simultaneous quantification of the major bile acids in bear bile powder in its quality assessment and control. METHODS: The chromatographic separation was carried out on Agilent SB-C18 column (4.6 mm × 250 mm, 5 μm) at 40℃ using 10 mmol·L-1 ammonium acetate solution and acetonitrile as the mobile phase at a flow rate of 1.0 mL·min-1. The detection was performed using evaporative light scattering detector at drift tube temperature of 110℃ and nitrogen flow rate of 2.8 L·h-1 with 1 as the gain. RESULTS: The standard curves of tauroursodeoxycholic acid and taurochenodeoxycholic acid showed good relationship in the ranges of 0.087 2-0.348 8 and 0.076 0-0.342 0 mg·mL-1, respectively. The average recoveries were 101.9% (RSD was 1.9%) and 102.2% (RSD was 1.5%), respectively. CONCLUSION: The method is simple, rapid, and accurate. The analysis of the result showed that the method could be used i the quality assessment and control of bear bile powder.