Simultaneous determination of five compounds in extract of penthorum chinense pursh. using quantitative analysis of multi-components by single-marker / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a method of quantitative analysis of multi-components by single marker(QAMS) for simultaneous determination of five compounds in extract of Penthorum chinense Pursh. METHODS: A HPLC method was established on an Agilent ZORBAX SB-C18column(4.6 mm×250mm, 5μm). The mobile phase was acetonitrile-0.2% formic acid aqueous solution. Gradient elution was performed at a flow rate of 1.0 mL·min-1. The column temperature was 30℃ and the detection wavelength was set at 280 nm. Pinocembrin-7-0-β-D-glucoside was selected as the internal reference substance, and the relative correlation factors (RCF) of gallic acid, brevifolin carboxylic acid, 2, 6-dihydroxyacetophenone-4-0-β-D-glucoside, quercetin-3-0-β-D-xyloside and quercetin-3'-0-α-L-rhamnoside were determined by HPLC. The contents of the six components were determined by both QAMS method and external standard method and the results were compared. The accuracy and feasibility of the new method were validated by QAMSmethod and external standard method. RESULTS: No significant differences were observed in the determination results of five constituents except brevifolin carboxylicacid in 21 batches of extract of P. chinense by QAMS method and external standard method. CONCLUSION: The QAMS method is feasible and suitable to determine the contents of five constituents in the extract of P. chinense.