Effect of no-ginsenoside argininyl-fructoyl-glucose in red ginseng on mice's fatigue and immunizition / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the preliminary mechanism of no-ginsenoside small molecular component AFG (argininyl-fructoylglucose) in red ginseng to mice's immune and anti-fatigue by forced swimming. METHODS: Massive AFG was separated from red ginseng and was made powder (RM) by vacuum freeze drying machine. ICR mice were randomly divided into 4 groups; the control group (CG), low dose group (RM-LG, 100 mg·kg-1), medium dose group (RM-MG, 200 mg·kg-1), high dose group (RM-HG, 400 mg·kg-1), forced swimming test was made after continuous intragastric administration 28 d, and the mice's weight was tested; organ index, time of forced swimming, volume of lactic acid (LD), urea nitrogen (BUN), hepatic glycogen (Gly), antioxidant enzyme in serum, ability of spleen lymphocyte proliferation, CD3, CD4, CD8 levels in spleen lymphocyte, releasing volume of immune factor were determined. RESULTS: Compared with control group, weight and organ index of mice aren't significant difference in treatment group; the force swimming time of treatment group is significantly increased (P<0.01); volume of LD, BUN and MDA in serum of treatment group are significantly reduced (P<0.01), volume of Gly, glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in serum of treatment group are significantly increased (P<0.01); releasing volume of IL-2 and IgG in serum of treatment group are significantly increased (P<0.01); ability spleen lymphocyte proliferation and CD3, CD4, CD8 levels in spleen lymphocyte are significantly increased (P<0.05) at treatment group. CONCLUSION: AFG has the effect of anti-fatigue and improving immunity in mice.