Characteristics of triacylglycerols composition of medium/long chain structured triacylglycerols by ultraperformance convergence chromatography combined with a quadrupole time-of-flight mass spectrometry / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a method suitable for rapid and accurate analysis of triacylglycerols in medium/long chain structured triacylglycerols by ultraperformance convergence chromatography combined with a quadrupole time-of-flight mass spectrometry. METHODS: The separation was performed at 28℃ on a Waters Acquity UPCC system by anHSS C18 SB column (3.0 mm×150 mm, 1.8 μm) with gradient elutionof CO2 and methanol acetonitrile (73, V/V), the flow rate was 1.2 mL·min-1, the back pressure was 117.2 MPa, and methanol containing 0.1% formic acid and 2 mmol·L-1 ammonium formate was used as supplement solvent. Positive ion electrospray ionisation (ESI) mode and MS technology were used in which two separate scan functions were programmed for the MS acquisition. The qualitative analyses were carried out by using precise mass information of parent ions and product ions. RESULTS: A total of 52 triacylglycerols were obtained and their stuctures were identified by analyzing the precise mass information of their parent ions and product ions respectively. CONCLUSION: The method is simple, highly accurate, and environmental friendly with good resolution, and can be used for rapid and accurate analysis of medium/long chain triacylglycerols excipients.