Rapid identification of chemical constituents of folium hibisci mutabilis by UHPLC-Q-TOF-MS/MS / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1162-1168, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-859032
ABSTRACT
OBJECTIVE:
To qualitatively analyze the chemical constituents of Folium hibisci Mutabilis by high performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (UHPLC-Q-TOF-MS/MS).METHODS:
The separation was performed on a UHPLC Welch C18 column (2.1 mm×100 mm, 1.7 μm), with acetonitrile-0.1% formic acid as the mobile phase for gradient elution; ESI ion source was used; the data was collected in a negative ion mode. The chemical components of Folium hibisci Mutabilis were identified by analyzing the retention time, exact relative molecular mass, and cleavage fragments of MS/MS.RESULTS:
A total of 73 compounds were identified in Folium hibisci Mutabilis, including 42 flavonoids, 15 triterpenoids, 11 organic acids, 3 coumarins, and 2 other kinds of compounds by UPLC-Q-TOF-MS.CONCLUSION:
LC-MS/MS can identify the chemical components of Folium hibisci Mutabilis in a simple, which would provide a foundation for further exploration of the effective substances of Folium hibisci Mutabilis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS