Preparation of mangiferin-loaded transfersomes and its transdermal delivery characteristics / 中国药学杂志

ABSTRACT

OBJECTIVE: To prepare mangiferin transfersomes and investigate its transdermal delivery characteristics. METHODS: Mangiferin transfersomes were prepared by the method of film-dispersion, the in vitro percutaneous penetration study was conducted in the modified Franz diffusion cell, the distribution of transfersomes in skin was investigated by fluorescent tracer method, and the rat back airbag inflammation model was used to preliminarily evaluate the anti-inflammatory effect of mangiferin transfersomes with prostaglandin E2 (PGE2) content as the indicator. RESULTS: The average particle size of mangiferin transfersomes was (84.50 ± 5.26)nm, the polydispersity index (PDI) was (0.21 ± 0.012), the Zeta potential was(-10.83 ± 0.66)mV, the encapsulation efficiency (EE) was (64.07 ± 2.10)%, and the deformability was (20.00 ± 0.30)%; the cumulative permeation quantities in 24 h and intradermal retention of mangiferin transfersomes were (313.67 ± 22.62) and (60.34 ± 8.10) μg · cm-1, respectively. Fluorescent tracer method showed that the fluorescence intensity of FITC transfersomes in the inside of skin was stronger than that of FITC solution at 8 h. Anti-inflammatory test showed that the PGE2 contents in the middle and high dose mangiferin transfersomes groups decreased significantly. The anti-inflammatory effect of the high dose mangiferin transfersomes was even close to that of compound dexamethasone cream. CONCLUSION: Transfersomes can promote the percutaneous penetration of mangiferin, increase its intradermal retention, and enhance the anti-inflammatory effect of mangiferin significantly.