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Simultaneous determination of ginsenoside Rg1, Re, Rb1 and Jujuboside A, B in Renshen Jianpi pellets by HPLC / 中国药学杂志
Chinese Pharmaceutical Journal ; (24): 751-756, 2016.
Article in Chinese | WPRIM | ID: wpr-859128
ABSTRACT

OBJECTIVE:

To establish an HPLC method for simultaneous determination of ginsenoside Rg1, Re, Rb1 and jujuboside A, B in Renshen Jianpi Pellets (RSJPW).

METHODS:

The determination was conducted on an Eclipse XDB C18 column (4.6 mm × 250 mm, 5 μm) with the mobile phase of acetonitrile (A)-water (B) gradiently eluted at the following flow rates 0-90 min, 1.0 mL · min-1; 90-130 min, 1.0-0.5 mL · min-1; 130-140 min, 0.5 mL · min-1. And the column temperature was maintained at 35℃; the detection wavelength was set at 203 nm.

RESULTS:

The calibration curves of ginsenoside Rg,, Re, Rb, and jujuboside A, B were in good linearity over 0.32-2.24 μg (r=0.9982), 0.16-1.12 μg (r=0.995 3), 0.32-2.24 μg (r=0.9996), 0.16-1.12 μg (r=0.9915), 0.08-0.56 μg (r=0.9996). The corresponding average recovery rates were 97.18%, 97.62%, 98.79%, 98.48%, 94.51%; the standard deviations were 1.10%, 0.98%, 0.34%, 1.09%, 1.88%, respectively.

CONCLUSION:

The established HPLC method is accurate, reliable and reproducible, which can be used as a reference for the quality control of RSJPW.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Pharmaceutical Journal Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Pharmaceutical Journal Year: 2016 Type: Article