Bauhinia championii flavones allievate cardiomyocytes hypoxia/reoxygenation injury via inhibiting necroptosis / 中国药学杂志

ABSTRACT

OBJECTIVE: To observe the effect and mechanism of Bauhinia championii flavones (BCF) on anti-hypoxia/reoxygenation injury in cardiomyocytes via inhibiting necroptosis. METHODS: The cardiomyocytes hypoxia/reoxygenation injury model was developed and pretreated with Bauhinia championii flavones. ELISA was used to evaluate the contents of TNF-α, and the activities of T-AOC were determined by ultraviolet spectrophotometric method. The protein expression of RIPK3 was observed by Western-blotting, and the necroptosis rate was determined by using Annex v-FITC/PI double staining. RESULTS: Compared with model group, Bauhinia championii flavones pretreatment alleviated cardiomyocytes injury, increased T-AOC level, decreased the activity of TNF-α, down-regulated the expression of RIPK3, and inhibited cardiomyocytes necroptosis (P<0.05). It had synergistic effect when combined BCF with necrostatin-1 (P<0.05). CONCLUSION: BCF can inhibit necroptosis and has protective effects on hypoxia/reoxygenation injury, which are associated with increasing the level of T-AOC, down-regulating TNF-α and RIPK3, and decreasing the cardiomyocytes necroptosis rate.