Simultaneous determination of five active compounds in wild and culture materials of Rhodiola crenulata by RP-HPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a method for simultaneous determination of five components in wild and tissue-culture materials of Rhodiola crenulata. METHODS: The determination was performed on a Thermo-C18 column (4.6 mm×250 mm, 5 μm) with mobile phase composed of methanol-water (3268) at a flow rate of 0.8 mL·min-1. The injection volume was 10 μL, column temperature was set at 30℃, and the detection wavelength was set at 277 nm at 0-13 min and 250 nm at 13-60 min. RESULTS: There were linear relationships between the peak areas and contents of salidroside, p-tyrosol, rosarin, rosavin and rosin in the ranges of 2.80-280.00 (r=0.9998), 2.80-280.00 (r=0.9997), 1.20-120.00 (r=0.9996), 1.60-160.00 (r=0.9997) and 1.20-120.00 μg·mL-1 (r=0.9997), respectively. The extraction recoveries varied from 99. 32% to 100.45%. CONCLUSION: The established method is simple and accurate for quality control of wild and tissue-culture materials of Rhodiola.