A fast identification method of notoginseng radix etrhizoma based on molecular signature / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a fast identification method of Panax notoginseng, molecular signature which can be used as fast authentication of P. notoginseng were screened according to comparison results of 248 Panax ITS2 sequences from GenBank and 46P. notoginseng ITS2 sequences from this study. METHODS: ITS2 Sequences obtained in this study were assembled using Codoncode Aligner, and then were imported to MEGA 6.0 together with Panax ITS2 sequences from GenBank. The comparison was conducted using MUSCLE method and then unique molecular signature of P. notoginseng was found in this comparison results. RESULTS: The length of ITS2 sequences of P. notoginseng was 230 bp, and the aligned ITS2 sequences of Panax was 258 bp. Based on one SNP site of P. notoginseng, one unique molecular signature was developed, 5'-AACCCATCAT TCCCTCGCGG GAGTCCATGC GGAGG-3'. If unknown samples contained the signature sequence after amplification, it would be identified to be Panax notoginseng and vice versa. CONCLUSION: The molecular signature obtained from this study can be used for fast authentication of varying kinds of P. notogin sengcrude drugs and powders and authentication of mixed samples.