Chemical constituents in chloroform extraction of Tetrastigmatis hemsleyani diels et Gilg and their antitumor activities / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the chemical constituents in chloroform extraction of Tetrastigmatis hemsleyani diels et. Gilg and their antitumor activities. METHODS: Various chromatography techniques such as column chromatography on silica gel, Sephadex LH-20 and preparative TLC were used to isolate and purify the compounds. Their structures were identified by 1H-NMR, 13C-NMR and MS. Their antitumor activities was tested by MTT method. Moreover, the other compounds of chloroform extraction were detected by GC-MS. RESULTS: Six compounds were isolated by classic chromatography and identified as β-sitosterol(1), 4-hydrox-y-3-methoxybenzaldehyde(2), oleanolic acid (3), 5-hydroxymethyl furfural(4), azelaic acid(5), vanillic acid(6). Twenty-two compounds were identified by GC-MS. CONCLUSION: Compounds 2-6 are isolated from this plant for the first time. Compounds 1 and 3 shows strong cytotoxic activities against Hela229 with IC50 values of 40.78, 25.69 μg · mL-1, respectively. Compound 3 also showed strong cytotoxic activities against with IC50 values of 69.87 μg · mL-1. The result proved that antitumor activity of chloroform extraction of Tetrastigmatis hemsleyani diels et. Gilg is due to the contribution of multi-components.