Treatment of Helicobacter pylori infection mice with Anti-H. pylori rFlaA IgY-sucralfate EC microcapsules / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the efficacy of Anti-H. pylori rFlaA IgY-sucralfate ethyl cellulose(EC) microcapsules on Helicocacter pylori infection in mice. METHODS: After dealing with sucralfate, the rFlaA IgY microcapsules were prepared with EC by organic solvent evaporation method. The protective effect of sucralfate at different concentrations of pepsin in different pH on the rFlaA IgY activity was also evaluated. Treating the mice with the microcapsules by oral at various dosages (0.8, 1.6, 3.2 and 6.4 mg · g-1 · d -1), the anti- H. pylori serum of the mice and the urease of the gastric mucosa were detected to evaluate the clearance rate of H. pylori by ELISA and rapid urease test. RESULTS: The results showed that under simulated gastric juice with common pepsin concentration (0.02 mg · mL-1), the relative activity of the IgY treated with 40% sucralfate can maintain 82.14% for 8 h at pH 3.0 and 63.28% at pH 2.0, it can only keep 21.52% at pH 1.0. While under the simulated gastric juice with high pepsin concentration (0.04 mg · mL-1), the relative activity of the IgY can maintain 77.87% with 40% sucralfate at pH 3.0, 57.76% at pH 2.0 and 22.79% at pH 1.0. The entrapment efficiency of the EC microcapsules was 83.79%. The release rate of the EC microcapsules was 95.92% in 5 h. After treatment with rFlaA IgY EC microcapsules, the levels of anti-H. pylori antibody were significantly decreased in each group. The clearance rate of H. pylori at dosage of 3.2 mg · g-1 · d-1 was 80%, the rFlaA IgY at dosage of 6.4 mg · g-1 · d-1 can completely clear the H. pylori in mice gastric mucosa. CONCLUSION: The 40% sucralfate can protect IgY in the simulated gastric juice. The rFlaA IgY EC microcapsules have high entrapment efficiency and sustained-release effect in vitro as well as good clearance effect of H. pylori infection by oral administration.