The effect of phloretin on rBMECs uptake of ginsenoside Rb1 / 中国药学杂志

ABSTRACT

OBJECTIVE: To evaluate the effect of phloretin on the uptake of ginsenoside Rb1 by the cell model, primary rat brain microvascular endothelial cells, (rBMEC) in vitro. METHODS: Experiments were divided into two groups, ginsenoside Rb1 with and without the addition of phloretin intervention. The cellular uptake of ginsenoside Rb1 was determined by LC-MS/MS. Protein content in cultured cells was measured by the method of BCA. RESULTS: The uptake amount of ginsenoside Rb1 in rBMEC with phloretin was significantly lower(P < 0.05). CONCLUSION: The uptake of ginsenoside Rb1 by rBMEC is significantly inhibited by phloretin.