Investigation of symbiotic Armillaria species with Chinese traditional medicinal fungus Polyporus umbellatus / 中国药学杂志

ABSTRACT

OBJECTIVE: To identify symbiotic Armillaria species with Polyporus umbellatus. METHODS: Armillaria was cultured on PDA paltes. To determine whether the strains were pure culture, the internal intergenic spacer (IGS) region were sequenced. Furthermore, the NCBI BLAST program was used to search similar sequences in the GenBanksequence database for the IGS sequence of the species on homology, and a phylogenetic tree was constructed based on neighbor-joining method. RESULTS: All isolates were similar in colony morphology and grew well in PDA medium with well-developed rhizomorphs. The isolated 22 strains were pure culture of the fungus. Phylogenetic analysis based on IGS sequence indicated that the symbiotic Armillaria species with Polyporus umbellatus belonged to A. cepistipes, A. gallica, A. ostoyae, and A. mellea. CONCLUSION: In the present study, phylogenetic analysis based on molecular method was carried out for the symbiotic Armillaria species with P. umbellatus for the first time. The results suggest that Armillaria species have no specificity with P. umbellatus, which is different from the previous reports. Further research will be done on the growth of P. umbellatus affected by different kinds of Armillaria species.