Effect of pioglitazone on oxidative stress and islet function in rats suffered from chronic intermittent hypoxia / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate effects of pioglitazone on oxidative stress and islet function in rats suffered from chronic intermittent hypoxia. METHODS: A chronic-intermittent hypoxia model in rats was established. Forty-eight male SD rats were randomly assigned into three experimental groups (n=16/group), normoxia control group, chronic intermittent hypoxia model group, and pioglitazone intervention group. Rats in NC group were raised normally, while rats in CIH and pioglitazone group were exposed to alternative cycles of O2 and N2. Rats in pioglitazone group were fed with pioglitazone (10 mg·kg-1·d-1) by gavaged, and both the CIH and NC groups were treated with isovolume normal saline solution (NS). The rats were sacrificed at 4 and 8 weeks and changes were detected about tumor necrosis factora, malondialdehyde, glutathione peroxidase, 8-iso-prostaglan-din F2α, superoxide dismutase in serum as well as HOMA-β and the expression of nuclear transcription factor-κB mRNA in pancreas tissue. RESULTS: Compared with NC group, the level of TNF-α, MDA, 8-iso-PGF2α in serum and expression of NF-κB mRNA increase, GSH-Px, SOD and HOMA-α declines in CIH group (P0.05). Compared with CIH group, the level of TNF-α, MDA and 8-iso-PGF2a in serum and expression of NF-κB mRNA in pancreas tissue decline, GSH-Px, SOD and HOMA-β rises in pioglitazone rats (P<0.05 or P<0.01), and those detections in pioglitazone 8 group show more remarkable changes than rats in pioglitazone 4 group (P<0.05 or P<0.01). CONCLUSION: The condition of CIH results in oxidative stress and islet β cell dysfunction of rats. Pioglitazone can degrade oxidative stress level through inhibiting NF-κB translocation and improve islet β cell function in chronic intermittent hypoxia rats, dependent of time to some degree.