Intervention effect of ethanol extract of oldenlandia diffusa on TGF-β1-induced epithelial mesenchymal transformation of human lung adenocarcinoma cell H358 / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate whether the ethanol extract of Oldenlandia diffusa (EEHD) have impact on the TGF-β1-induced epithelial mesenchymal transformation (EMT) of human lung adenocarcinoma cell H358 in vitro. METHODS: The cell model to study EMT of H358 cells was established with 5 ng·mL-1 TGF-β1 for 24 h. Then, after being mixed with low, medium and high concentration of EEHD respectively for 48 h, H358 cells were collected for detection of protein and mRNA expression of epithelial marker E-cadherin and mesenchymal cell marker vimentinby Western-Blot and quantitative PCR. RESULTS: The concentration of 5 ng·mL-1 TGF-β1 can successfully induce the transformation from epithelialphenotype to mesenchymal phenotype. The concentration of EEHD is positively correlated with increasing expression of E-cadherin, while negatively correlation with the decreasing expression of vimentin. CONCLUSION: The EEHD have partially reversal effect of epithelial mesenchymal transformation on lung adenocarcinoma cell induced by TGF-β1.