Simultaneous determination of six constituents in rukuaixiao granules by UHPLC-MS/MS / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 629-633, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-859407
ABSTRACT
OBJECTIVE:
To establish a UHPLC-MS/MS method for simultaneous determination of tangertin, hesperetin, 5-demethylnobiletin, nobiletin, vitexin, and narirutin in Rukuaixiao granules.METHODS:
The analysis method was performed on a Waters ACQUITY UPLC® BEH C18 eolumn (2.1 mm×50 mm, 1.7 μm) with gradient elution of acetonitrile (containing 0.1% formic acid)-0.1% formic acid at a flow rate of 0.6 mL·min-1, and the column temperature was set at 50℃. Electrospray ionization (ESI) source with positive mode and the detector with multiple reaction monitoring (MRM) mode were adopted to determine the analytes by mass spectormeter.RESULTS:
Satisfactory linearities were obtained for the six constituents in the investigated ranges (r>0.9958) The lower limit of quantitation (LOQ) was 0.80, 1.10, 1.30, 0.66, 2.00, 7.10 and 0.70 ng·mL-1, respectively. All the recoveries of samples were between 95.78%-101.35% with RSDs less than 2.1%.CONCLUSION:
The method is simple, rapid, sensitive, and highly reproducible. It can be applied to the quality control of Rukuaixiao granules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS