Study on the flavonoid glycosides from the herb of clematis connata DC / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the flavonoid glycosides in Clematis connata DC. METHODS: Colum chromatography with different materials, such as RP-18, Sephadex LH-20 and silica gel, and semi preparative high performance liquid chromatography were used to isolate and purify the chemical constituents. Their structures were identified by spectroscopic analysis. RESULTS: Eleven flavonoid glycosides were isolated and identified as kaempferol-3-O-β-D-glucopyranosyl methyl ester (1), isovitexin (2), kaempferol-3-O-β-D-glucopyranoside-7-α-L-rhamnopyranoside (3), kaempferol-3-O-α-L-rhamnopyrano-side-7-α-L-rhamnopyranoside (4), kaempferol-3-O-α-L-(4-O-acetyl) rhamnopyranoside-7-α-L-rhamnopyranoside (5), kaempferol-3-O-(2-β-D-glucopyranosyl)-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside (6), genistein-7-O-β-Z)-apiofurano-syl-(1→6)-O-β-D-glucopyranoside(7), lanceolarin(8), quercetin-3-O-β-D-(6″-n-butyl) glucuronide(9), quercetin-3-O-β-D-glucopyranosyl methyl ester(10), and isoorientin (11). CONCLUSION: Compounds 1-11 are isolated from Clematis connata DC for the first time.