Simultaneous determination of camphor, isoborneol, L-borneol, β-caryophyllene and xanthoxylin in aifen by GC and hierarchical cluster analysis / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a GC method for simultaneous determination of camphor, isoborneol, L-borneol, β-caryo-phyllene and xanthoxylin in aifen, combined with hierarchical cluster analysis. METHODS: The GC analysis was carried out on HP-5 capillary column (30 m × 0.32 mm, 0.25 μm). Temperature programs; 90℃ (hold 2 min) programmed to 100℃ at 4℃ · min-1, then programmed to 160℃ (hold 6 min) at 20℃ · min-1. The detector was FID with 240℃. The inlet temperature was set at 240℃. The carrying gas was high-purity nitrogen (3.0 mL · min-1), split ratio was 501, injection volume was 0.5 μL. Cluster analysis was performed by SPSS16.0 software. RESULTS: The methodology validation for the assay of camphor, isoborneol, l-horneol, β-caryophyllene and xanthoxylin presented that they were in good liner correlation in the ranges of 0.02-0.32 (r=1.000 0), 0.01-0.08(r=1.000 0), 0.20-7.95(r=1.000 0), 0.01-0.40(r=1.0000), 0.08-0.67 mg · mL-1 (r=0.999 8), with the average recoveries (n=9) of 101.04% (RSD=1.04%), 99.08% (RSD=1.50%), 98.76% (RSD=1.67%), 99.85% (RSD=1.97%), 97.74% ( RSD=1.65%), respectively. The 24 batches of sample analyzed can be clustered into three classes according to the content of the five components. CONCLUSION: The developed method is simple, quick and accurate, which provides reference for the quality control of aifen.