Chemical constituents of whole plants of tetrastigma hemsleyanum and their antitumor activities / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the chemical constituents and antitumor activity of Tetrastigma hemsleyanum Diels et Gilg. METHODS: The compounds were isolated by various column chromatography. Their structures were identified by spectroscopic methods including 1H-NMR, 13C-NMR, MS, HR-MS, etc. The antiproliferative activity of the purified flavonoids compounds were evaluated by MTT assay against HepG2, HCT-8, and A549 tumor cells. RESULTS: Thirteen compounds were isolated and identified as β-sitosterol (1), palmitic acid (2), heptadecanoic acid (3), apigenin (4), quercitrin (5), kaempferitrin (6), kaempferol-3-O-neohes-peridoside (7), apigenin-6-C-β-D-glucopyranoside (8), apigenin-8-C-α-L-rhamnopyranosyl-(1-2)-β-D-glucopyranoside (9), apige-nin-8-C-β-D-glucopyranoside (10), apigenin-8-C-β-D-glucopyranoside-(1-4)-β-D-glucopyranoside (11), orientin (12), and isoorientin (13). CONCLUSION: Compounds 4-6 and 8-13 were isolated from T. hemsleyanum for the first time. The flavonoids of T. hemsleyanum showed inhibitory activity on HepG2, HCT-8, and A549 tumor cells.