Effect of BPEI/miRNA, PAMAM/miRNA and PEI/miRNA delivery system on the treatment of prostate cancer / 中国药学杂志

ABSTRACT

OBJECTIVE: To identify the possibility of branched polyethyleneimine (BPEI), polyamidoamine dendrimers (PAMAM) and linear polyethylenimine (LPEI) as gene vector for miRNA-15a and miRNA-16-1 gene therapy in prostate cancer. METHODS: The diameter and Zeta potential of complex were measured by Zetasizer Nano-ZS9, and its stability was also observed. The efficiency of transfection in vitro was detected by flow cytometer and the positively transfected cells were detected by fluorescence microscope. The inhibition of complex on LNCaP cell was determined by CCK8 assay. The expression of protein BCL-2, CCDN1 and Wnt3a were detected by Western blot method. RESULTS: BPEI, PAMAM and LPEI with miRNA formed nano-sized particles. The transfection rate of BPEI/miRNA-CY3 was significantly higher than that of PAMAM/miRNA-CY3 and LPEI/miRNA-CY3 at N/P ratio (P < 0.01). The protein level of BCL-2, CCDN1 and Wnt3a in BPEI, PAMAM and LPEI were all lower than the control group in LNCaP cells. CONCLUSION: With good stability and transfection rate, BPEI/miRNA, PAMAM/miRNA and LPEI/miRNA can be a promising nano-vector of miRNA transfer system. The complex can inhibit the expression of BCL-2, CCDN1 and Wnt3a gene specifically and efficiently, which may be used for prostate cancer treament.