Analysis of the metabolites of genistin and enzyme kinetics of genistin in rat liver microsome / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 797-801, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-859470
ABSTRACT
OBJECTIVE:
To characterize the metabolism of genistin and study its enzymatic kinetics in rat liver microsome by HPLC-MS.METHODS:
Genistin was incubated with rat liver microsomal incubation system. HPLC-MS method was used to characterize the metabolites. A metabolite generation method was established for quantitative analysis of genistein with sulfamethoxazole as internal standard. The enzyme kinetics parameters Vmax and Km was calculated by the GraphPad Prism 5.0 software.RESULTS:
The metabolites in vitro incubation system was identified as genistein. The optimal time in rat liver microsomes incubation time of 40 min, the optimal protein concentration of 1 mg · mL-1, a substrate concentration of 50 μmol · L-1. The enzyme kinetics parameters of genistein were as follows; Vmax=(0.1042 ± 0.0033) μmol · min-1 · mg(pro)-1, Km=(28.96 ± 2.80) μmol · L-1.CONCLUSION:
The results indicate that genistin can be metabolited as the form of hydroxylation in rat liver microsome. Metabolite generation method is a reliable and simple method for determination of kinetic parameters of hepatic microsomal enzymes, and enzyme kinetic parameters obtained of genistin provide important parameters for further study.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS