Stimulating effects of toad venom (CHANSU) in the rat hind paw / 中国药学杂志

ABSTRACT

OBJECTIVE: The stimulating effects of toad venom (Chansu) on rats were investigated in this study. METHODS: After treatment with toad venom (sc, 10 and 50 mg · mL-1), foot swelling and foot-ground reaction force were observed to reflect the inflammation and pain, respectively. The levels of inflammatory mediators (PGE2, PGF2α, PGE1, and 8, 9-DiHETrE) were further determined using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). RESULTS: No raising foot was observed in the normal rats group, but this foot-ground reaction force was significant in the toad venom 10 and 50 mg · mL-1 groups. The number of raising foot after toad venom treatments were (14 ± 5) and (99 ± 8) times in 10 min (P 2 and PGF2α, and reduced the relative content of anti-inflammatory PGE1 and 8, 9-DiHETrE in the rat hind-paw. CONCLUSION: Toad venom can dose-dependently produce stimulating effects in the rat paw.